Vascular Effects of Ezetimibe/Simvastatin and Simvastatin on Atherosclerosis

Condition(s) targeted: Atherosclerosis

Intervention: Simvastatin (Zocor) (Drug); 10mg/10mg of Ezetimibe/Simvastatin (Vytorin) (Drug)

Phase: Phase 4

Status: Recruiting

Sponsored by: National Cheng-Kung University Hospital

Official(s) and/or principal investigator(s):
Jyh-Hong Chen, MD, PhD, Principal Investigator, Affiliation: National Cheng-Kung University Hospital

Overall contact:
Jyh-Hong Chen, MD, PhD, Phone: 886-6-2353535, Ext: 2389, Email: jyhhong@mail.ncku.edu.tw

Multiple clinical trials, using 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase inhibitors (statins), have shown benefit in the primary and secondary prevention of atherosclerotic complications. However, till now, there is an incomplete understanding of all the mechanisms of the biologic effects of statins beyond LDL cholesterol (LDL-C) reduction, but there is accumulating evidence that the Rho-GTP/Rho-Kinase pathway (Rho/Rho-K) plays an important role and may be a strategic therapeutic target in cardiovascular diseases. With similar LDL-C reduction ability, the availability of Ezetimibe offers the potential to begin to address the question whether some of the benefits conferred by statins may accrue independently of their effects on LDL-C lowering. A better understanding of the role of the Rho/Rho-kinase signaling pathway in the pathogenesis of atherosclerosis in human is essential. Inhibition of Rho/Rho-kinase by statins may explain some of the biological beneficial effects of statins observed in clinical trials. This study aims to translate into patients important experimental discoveries regarding the initiation of inflammation in atherosclerosis in an attempt to improve upon the present treatment of cardiovascular diseases.

Official title: Rho-Kinase in Patients With Atherosclerosis: Effects of Statins A Randomized Clinical Trial Comparing Ezetimibe/Simvastatin and Simvastatin

Study design: Basic Science, Randomized, Single Blind (Outcomes Assessor), Parallel Assignment, Efficacy Study

Primary outcome: changes in the lipid profile change and Rho-kinase expression and activity

Secondary outcome: correlation between changes in Rho-kinase expression and activity with the changes in LDL-C, hsCRP and BAFMD

Detailed description: Study Design:

A single-blind controlled trial with two arms will be conducted at National Chen-Kung University Hospital (NCKUH). We will screen subjects with stable atherosclerosis to complete enrollment of 40 subjects in the study (see inclusion and exclusion criteria section below). A central pharmacist at NCKUH will randomize the patients to 40mg or Simvastatin (n=20) or 10mg/10mg of Ezetimibe/Simvastatin (n=20) for 28 days. If the patient is already on a statin a two-week washout period will be 2 weeks prior to trial initiation.

Primary Outcomes and measurement:

The primary outcomes are the mean changes in the Rho-kinase expression and activity in leukocytes in response to 40mg or Simvastatin (n=20) or 10mg/10mg of Ezetimibe/Simvastatin (n=20) over 28 days.

Secondary Outcomes and measurement:

The secondary outcomes are the correlation between the mean changes in Rho-kinase expression and activity in leukocytes and vascular tissue with the mean changes in LDL-C, hsCRP, and BAFMD, as well as its relation with clinical characteristics.

Subjects:

Participants will be recruited from the ambulatory clinics at the NCKUH Clinic.

Inclusion Criteria:

1. Male or female subjects aged 40 to 80 years

2. Documented stable atherosclerosis by angiography or vascular ultrasound (more that 20% luminal narrowing), peripheral arterial disease or type 2 diabetes mellitus (coronary

heart disease (CHD) risk equivalent - Adult Treatment Program (ATP)-III guidelines)

3. LDL-cholesterol >100mg/dL (indication to treat with statin)

4. Written informed consent

5. Primary care physician authorization letter to participate in the study.

Exclusion criteria:

1. Inability to give consent

2. Pre-menopausal women

3. Current use of antibiotics, anti-inflammatory or immunosuppressant drugs

4. History of LFT >2 times the upper normal limit

5. History of myopathy / myositis or CPK > 10 times the upper normal limit

6. CPK above normal limits at study onset

7. Any evidence of inflammatory, infectious or neoplastic disease

8. History of CABG, PCI or acute ischemic syndrome in the preceding 3 months.

Minimum age: 40 Years. Maximum age: 80 Years. Gender(s): Both.

Criteria:

Inclusion Criteria:

- Inclusion Criteria:

1. Male or female subjects aged 40 to 80 years

2. Documented stable atherosclerosis by angiography or vascular ultrasound (more that 20% luminal narrowing), peripheral arterial disease or type 2 diabetes

mellitus (coronary heart disease (CHD) risk equivalent - Adult Treatment Program

(ATP)-III guidelines)

3. LDL-cholesterol >100mg/dL (indication to treat with statin)

4. Written informed consent

5. Primary care physician authorization letter to participate in the study.

- Exclusion criteria:

1. Inability to give consent

2. Pre-menopausal women

3. Current use of antibiotics, anti-inflammatory or immunosuppressant drugs

4. History of LFT >2 times the upper normal limit

5. History of myopathy / myositis or CPK > 10 times the upper normal limit

6. CPK above normal limits at study onset

7. Any evidence of inflammatory, infectious or neoplastic disease

8. History of CABG, PCI or acute ischemic syndrome in the preceding 3 months.

Jyh-Hong Chen, MD, PhD, Phone: 886-6-2353535, Ext: 2389, Email: jyhhong@mail.ncku.edu.tw

National Cheng Kung University Hospital, Tainan 704, Taiwan; Recruiting
Jyh-Hong Chen, MD, PhD, Phone: 886-6-2353535, Ext: 2389, Email: jyhhong@mail.ncku.edu.tw
Ping-Yen Liu, MD, PhD, Phone: 886-6-2353535, Ext: 2389, Email: larry@mail.ncku.edu.tw
Jyh-Hong Chen, MD, PhD, Principal Investigator
Ping-Yen Liu, MD, PhD, Sub-Investigator
Yen-Wen Liu, MD, Sub-Investigator
Li-Jen Lin, MD, Sub-Investigator

Starting date: September 2007
Ending date: February 2008
Last updated: November 16, 2007