Cabergoline Reduces OHSS
Information source: Instituto Valenciano de Infertilidad, Spain
Information obtained from ClinicalTrials.gov on June 20, 2008
Link to the current ClinicalTrials.gov record.
Condition(s) targeted: Ovarian Hyperstimulation Syndrome
Intervention: Cabergoline (Drug); MR (Procedure); Ultrasound (Procedure); Blood Analysis (Procedure)
Phase: Phase 3
Status: Completed
Sponsored by: Instituto Valenciano de Infertilidad, Spain Official(s) and/or principal investigator(s):
Antonio Pellicer, MD, Study Director, Affiliation: IVI VALENCIA
Summary
The present study was designed to provide clinical confirmation of Cb2’s value as a new
approach in the prevention of increased vascular permeability and hemoconcentration, both
signs of OHSS in humans, and in order to explore its mechanism of action. To this end, a
prospective, randomized, placebo-controlled study was designed in which Cb2 was employed in
women at risk of OHSS after gonadotropin administration for ART. Simultaneously, ovarian
perfusion was assessed in these patients using MR pharmacokinetic modeling.
Clinical Details
Official title: Dopamine Agonist Cabergoline Reduces Hemoconcentration and Ascites in Hyperstimulated Women Undergoing Assisted Reproduction.
Study design: Prevention, Randomized, Double-Blind, Placebo Control, Parallel Assignment, Efficacy Study
Detailed description:
Patients and Study design This study was performed in 82 oocyte donors between April 2004 and
July 2006. The study protocol was approved by our institution’s Ethical Committee and all
participants signed a written consent form. The protocol for COH has previously been
described (23). Only patients at risk of developing OHSS were included. The definition of
risk was the development of 20-30 follicles >12 mm in diameter, and retrieval of >20
oocytes. Once the decision to administer hCG was taken, patients were immediately allocated
into two groups based on a computer randomization: the study group initially consisted of 41
patients, but 6 of these were discarded after randomization because <20 oocytes were
retrieved despite the development of >20 follicles >12 mm. Thus, a remaining total of 35
patients received one 0. 5 mg tablet of Cb2 daily for eight days. The control group was also
initially composed of 41 women. However, 7 of these did not meet the criteria of number of
oocytes retrieved, and 2 donors decided to withdraw themselves from the study. This left a
remaining total of 32 women, all of whom completed the study, and who received a placebo
tablet daily for 8 days. Women were monitored every 48 hours from the day of hCG (day 0)
until day 8.
On day hCG+4, in order to define ascites, and provided that there was a certain degree of
fluid in the pouch of Douglas after ovum pick-up, we employed transvaginal ultrasound (TVU)
to measure two major perpendicular diameters of fluid pockets in 15 donors who showed no risk
of OHSS. We observed 3. 5±2. 8 cm2 of fluid in the pelvis in normal conditions. Therefore, the
existence of ascites was confirmed when a pocket of peritoneal fluid >9 cm2 was observed when
the patient was in lithotomy position (with the gynecological table always at 45º from the
floor of the room), which is the result of the mean± 2 standard deviations (SD) of the value
found in non-OHSS candidates. TVU scans were performed by the same researcher (CA), who was
blind to the treatment to which the patient was submitted. A 6. 5 MHz vaginal probe (Voluson
730 Pro V, General Electric, Madrid, Spain) was employed for all TVU scans.
To evaluate the biochemical risk of hemoconcentration, we evaluated hemoglobin, hematocrit,
and leukocyte count. Moreover, renal (creatinine) and liver [transaminases: aspartate
aminotransferase (AST); alanine aminotransferase (ALT)] functions, and electrolytes (Na, K)
were analyzed to ascertain the severity of the syndrome.
Since all women undergoing ART experience a certain degree of discomfort and enlarged ovaries
(known as mild OHSS), we centered our attention on analyzing the incidence of moderate and
severe OHSS, which were identified according to our modified (24) classification of Golan et
al (25). Moderate OHSS was confirmed when a patient presented ultrasonographic evidence of
ascites, while diagnosis of severe OHSS required clinical evidence of ascites and/or
hydrothorax and breathing difficulties, or one of the following criteria: a) increased blood
viscosity due to hemoconcentration (hemoglobin ≥15 g/dl, hematocrit ≥45%, or leukocyte count
≥20,000/mm3); b) coagulation abnormality; c) diminished renal perfusion and function (serum
creatinine levels >1. 2 mg/dl); d)liver dysfunction: defined when transaminases (AST or ALT)
were >40 U/ml (24, 25).
Additionally, serum PRL levels were measured and adverse drug reactions recorded. An
end-of-study assessment was scheduled 7-10 days after the last dose of Cb2/placebo.
Moreover, in the first 8 patients included in the study, follicular fluid aspirates without
obvious blood contamination were collected, pooled and and mRNA extracted to quantify the
amount of Dp-r2 in human ovaries, employing two different molecular techniques.
To further objectively analyze changes in vascular permeability and fluid shifts,
confirmatory studies were performed on six women in the study group and four controls,
employing magnetic resonance (MR) as described below. Dynamic contrast-enhanced MR was
performed at three different stages of the study: at baseline, before gonadotropin
administration was initiated; just before hCG injection; and on day hCG+5, after oocyte
pick-up.
Eligibility
Minimum age: 18 Years.
Maximum age: 35 Years.
Gender(s): Female.
Criteria:
Inclusion Criteria:
- 18 - 35 years old healthy women, with risk of developing OHSS.
Exclusion Criteria:
- No risk of developing OHSS; < 20 oocytes retrieved.
Locations and Contacts
Additional Information
Starting date: April 2004
Ending date: July 2006
Last updated: February 26, 2007
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