Progestin Treatment for Endometrial Stromal Cells in Adenomyosis
Information source: National Taiwan University Hospital
Information obtained from ClinicalTrials.gov on November 03, 2008
Link to the current ClinicalTrials.gov record.
Condition(s) targeted: Endometriosis
Phase: N/A
Status: Recruiting
Sponsored by: National Taiwan University Hospital Official(s) and/or principal investigator(s):
Jehn-Hsiahn Yang, M.D., Principal Investigator, Affiliation: Department of Obstetrics and Gynecology, National Taiwan University Hospital
Overall contact:
Jehn-Hsiahn Yang, M.D., Phone: 886-2-2312-3456, Ext: 3210, Email: jhyang@ha.mc.ntu.edu.tw
Summary
Long term treatment of progestin has been demonstrated to have an inhibitory effect on
endometrial angiogenesis and the proliferation of endometrial stromal cells. As a result,
progestin is now widely employed in the treatment of endometrial cancer, endometrial
hyperplasia, and dysfunction uterine bleeding. In the treatment of adenomyosis, however, the
beneficial effect of progestin was limited. It might imply that the behavior of endometrial
cells in women with adenomyosis is different from that in women without adenomyosis.
Our previous study revealed that the expression of killer inhibitory receptors (KIRs) on NK
cells was decreased in eutopic endometrium in women with adenomyosis. It may be a
compensatory effect in which the NK cytotoxicity is activated in order to wipe out the
abnormal endometrial cells that might go out of the eutopic site of endometrium. It implies
that the formation of adenomyosis might be due to “abnormal” endometrial tissues, but not the
aberrant local immunological dysfunction in myometrium. This finding is compatible with
previous reports in which eutopic endometrium obtained from women with endometriosis or
adenomyosis was found to behave differently from endometrium in unaffected women.
In this study, we try to collect endometrial tissues from women with and without adenomyosis,
and then purify the endometrial stromal cells from endometrium. The endometrial stromal cells
are cultured for 8 days with the supplement of medroxyprogesterone (MPA) or danazol.
Quantification of IL-6 and IL-8 mRNA in endometrial cells, and the concentrations of IL-6 and
IL-8 in cultured media will be done with real time RT-PCR and ELISA respectively. The
expression of different cytokines of endometrial cells in response to progestin might be
further elucidated after our experiment.
Clinical Details
Study design: Natural History, Cross-Sectional, Defined Population, Prospective Study
Detailed description:
Eutopic endometrium was obtained and separated into single endometrial stromal cell (ESC) in
women with adenomyosis (study group) and without adenomyosis (control group).
After becoming pre-confluent (covering 80% of the culture well), ESC was cultured for 8 days
solely or with the addition of medroxyprogesterone (MPA) or danazol.
ELISA was done to measure IL-6, IL-8, and TNF-alpha concentrations of the culture media.
Real-time quantitative RT-PCR was done to measure IL-6, IL-8, and TNF-alpha RNA levels in
ESC.
Eligibility
Minimum age: 35 Years.
Maximum age: 50 Years.
Gender(s): Female.
Criteria:
Inclusion Criteria:
- women with adenomyosis
- at early- to mid-secretory phases
Exclusion Criteria:
- postmenopausal
- malignancy
Locations and Contacts
Jehn-Hsiahn Yang, M.D., Phone: 886-2-2312-3456, Ext: 3210, Email: jhyang@ha.mc.ntu.edu.tw
National Taiwan University Hospital, Taipei 100, Taiwan; Recruiting
Jehn-Hsiahn Yang, M.D., Phone: 886-2-2312-3456, Ext: 3210, Email: jhyang@ha.mc.ntu.edu.tw
Jehn-Hsiahn Yang, M.D., Principal Investigator
Additional Information
Starting date: July 2004
Ending date: April 2005
Last updated: September 9, 2005
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