- negative: PPD < 5mm (6 subjects)
- Negative Quantiferon test.
- Legal capacity to consent
- Subject had given written consent before his participation
- Subject accepting to participate in the second phase of the study with skin biopsies
(24 subjects with positive PPD)
- Females of childbearing potential using an effective method of contraception, for at
least 1 month before the beginning of the study and until 1 month after the end of
their participation
Exclusion Criteria:
- Known allergy to tuberculin
- History of severe skin reaction to tuberculin with vesicle, ulceration, necrosis
- History of active tuberculosis
- Latent tuberculosis (positive Quantiferon test)
- Contact with a person having or having had active tuberculosis in the previous 3
months
- Chronic disease non-stabilized under treatment
- Immunosuppressive therapy or corticosteroids within 1 month before PPD skin tests
- Non-steroid anti-inflammatory drugs within 1 week before PPD skin tests
- Application on study areas of topical drugs containing corticoids or
immunosuppressants within 1 week before PPD skin test
- Dermatological disease on study area
- Known allergy to local anesthetics
- Wound healing disorders
- Subject in an exclusion period or participating or planning to participate in another
biomedical research