Prospective Study of First-Line Antibiotic Therapy for Early-Stage Gastric MALT Lymphoma for Treatment Outcome

Condition(s) targeted: Gastric MALT Lymphoma

Intervention: Omeprazole, Amoxicillin, Clarithromycin (Drug)

Phase: N/A

Status: Recruiting

Sponsored by: National Health Research Institutes, Taiwan

Official(s) and/or principal investigator(s):
Jaw-Town , Lin, M.D., PHD, Principal Investigator, Affiliation: Taiwan cooperative oncology group
Li Tzong Chen, M.D., Ph.D., Principal Investigator, Affiliation: Taiwan cooperative oncology group

Overall contact:
Wei-lien Feng, RN,MSN, Phone: 886-2-8792-3311, Ext: 17644, Email: winnif@nhri.org.tw

1. The complete histological and molecular remission rate for antibiotics as 1st-line therapy for Hp-positive early-stage gastric lg- and hg-MALT lymphoma

2. The durability of complete histological remission after antibiotics

3. The usefulness of pattern of NF-kB and BCL-10 by IHC staining in prospectively predicting the Hp-dependence of gastric lg- and hg-MALT lymphoma

4. The frequency of t(11;18) translocation in gastric lg- and hg-MALT lymphoma in Taiwan.

5. The association between the CYP2C18/19 genetic polymorphisms and eradication of Hp infection after antibiotics.

Official title: Multicentre,Prospective Study of First-Line Antibiotic Therapy for Early-Stage Low-Grade and High-Grade Gastric Mucosa-Associated Lymphoid Tissue-Type Lymphoma and Potential Predicting Factor for Treatment Outcome

Study design: Treatment, Non-Randomized, Open Label, Historical Control, Single Group Assignment, Safety Study

Primary outcome: Hp eradication rate and complete histological rate

Secondary outcome: overall survival (OS)Relapse-free survival (RFS)

Detailed description: Background: Eradication of Helicobacter pylori (Hp) infection is well recognized as the initial therapy for early-stage low-grade gastric mucosa-associated lymphoid tissue-type lymphoma (lg-MALT lymphoma). On the other hand, high-grade transformed MALT lymphoma (hg-MALT lymphoma) is generally considered to arise from Hp-independent clones and thus to be unlikely to respond to antibiotic therapy. Our previous prospective studies have firstly demonstrated that 1st-line antibiotic therapy could achieve durable complete histological remission in two-third of Hp-positive stage IE hg-MALT lymphoma (Chen et al., J. Clin. Oncol., 2001), in which the long-term clinical outcomes were equivalent to those achievable in lg-MALT lymphoma (Chen et al. J Natl Cancer Inst, accepted). In addition, our laboratory studies have confirmed that t(11;18) translocation is associated with loss of Hp-dependence in lg-MALT lymphoma but infrequently found in high-grade tumors. We also found that nuclear translocation of NF-kB or BCL-10 (by immunohistochemical, IHC, staining) were useful markers to predict the Hp-dependence of both early-stage gastric hg- and lg-MALT lymphoma to antibiotic therapy (Kuo et al. JCO 2004 & Yeh et al. Blood 2005). In addition, recent data suggested cytochrome CYP2C18/19 genetic polymorphisms are associated with the metabolism of omeprazole, and thus the genotype of such enzymes might affect the efficacy of antibiotics for eradication of Hp infection.

Aims: A nationwide study to prospectively validate

1. The complete histological and molecular remission rate for antibiotics as 1st-line therapy for Hp-positive early-stage gastric lg- and hg-MALT lymphoma

2. The durability of complete histological remission after antibiotics

3. The usefulness of pattern of NF-kB and BCL-10 by IHC staining in prospectively predicting the Hp-dependence of gastric lg- and hg-MALT lymphoma

4. The frequency of t(11;18) translocation in gastric lg- and hg-MALT lymphoma in Taiwan.

5. The association between the CYP2C18/19 genetic polymorphisms and eradication of Hp infection after antibiotics.

Materials and Methods: Patients with newly, histologically proven stage IE / IIE-1 gastric lg- and hg-MALT lymphoma are eligible. Pre-treatment Hp infection status will be determined by histology, rapid urease test and serology. At time of registration, patients should agree to provide endoscopic biopsy specimen, including eight 4-mm histologic section for immunohistochemical study of NF-kB and BCL-10 and three 10-mm of section in eppendorf tube for RNA extraction and subsequent RT-PCR for t(11;18) translocation determination, which will be performed at the central laboratory. In addition, serum (from 5 mL of coagulated blood) as well as peripheral blood mononuclear cells (from 3 mL of heparized blood) will also collected before treatment for Hp-serology and CYP 2C18/19 genetic polymorphism detection, respectively. Hp-positive patients will receive 2-week of triple therapy, consisting of omeprazole, amoxicillin and clarithromycin (OAC regimen), and have first follow-up endoscopy 4 weeks later to determine the status of Hp infection and tumor response. Patients will then have sequential follow-up endoscopic examinations every 3 months until complete histological remission (CR) or disease progression; then every 6 months for complete responders. Patients with hg-MALT lymphoma who have stable or progressive disease after Hp eradication will immediately refer for systemic chemotherapy. CR was defined as regression of lymphoid infiltration to Wotherspoon’s score <2 on all pathological sections of endoscopic biopsy specimens. The predictive value of NF-kB, BCL-10 and t(11;18) for complete histological remission after Hp eradication will be determined.

Expected Results: 1st-line antibiotic therapy will achieve complete histologic remission in 70-80% of Hp-positive stage IE gastric lg-MALT lymphoma and in 50-60% of stage IE hg-MALT lymphoma. The objective histologic CR rate in stage IIE-1 disease may be 30-40% for low-grade tumor and 20-30% for high-grade ones. The sensitivity and specificity of NF-kB and BCL-10 positive nuclear staining by IHC and of t(11;18) in predicting the Hp-independence will be both 80 – 90%. Ten – twenty per cent of enrolled patients will have CYP2C19 m1/m1, m1/m2 or m2/m2 genotypes (considered as omeprazole poor metabolizer), and they might have higher Hp eradication rate than those extensive metabolizers .

Minimum age: N/A. Maximum age: N/A. Gender(s): Both.

Criteria:

Inclusion Criteria:

- The patients must have histologically confirmed primary gastric MALT lymphoma with or

without clustering large cells (extranodal marginal zone lymphoma, and diffuse large cell lymphoma with features of MALT by REAL/WHO classification, Harris NL et al. 1994).

- 1. 1 The diagnosis of primary gastric lymphoma must fulfill the criteria of

Dawson [38].

- (1)No enlargement of peripheral or mediastinal lymph node;

- (2)Peripheral blood smear revealing no leukemic or lymphomatous

abnormalities;

- (3)Predominant of alimentary tract lesions with any adenopathy

corresponding to accepted lymphatic drainage route; and

- (4)No involvement of liver or spleen except by extension of contiguous

disease.

- 1. 2 The diagnosis of MALT lymphoma will be made by histopathologists from

individual hospitals, in accordance with criteria defined by Isaacson et al. and Chang et al, and will be reviewed by the members of the TCOG Pathology Committee. This pathology review mechanism had been functioned well in the previous T1296 study (see J Natl Cancer Inst. 2005;97: 1345-53)

- 1. 3 The patient must have no prior chemotherapy or radiotherapy for his/her

gastric lg- or hg-MALToma.

- Patients must have evaluable disease by endoscopy and/or the nodal status by computed

tomography. Endoscopic ultrasonography (EUS) is mandatory to evaluate the depth of tumor infiltration and for status of perigastric lymph node enlargement.

- Patients must have documented H. pylori infection before treatment, which will be

evaluated by the following tests: histology, rapid urease test (CLO-test), C-13 urease breath test and serology.

- 3. 1 The following will be considered to have H. pylori infection: if any of

above 4 tests show positive result.

- Patients must have either stage IE or IIE-1 disease, according to an adaptation of

the Ann Abor staging system modified by Musshoff for primary extranodal lymphoma.

- 4. 1 Stage IE : lymphoma confined to the gastric wall without lymph node

involvement.

- 4. 2 Stage IIE : localized involvement of one or more GI site(s) on one side of

the diaphragm with lymph node involvement, any depth of lymphoma infiltration into the gut wall. 4. 21 Stage IIE-1: involvement of perigastric lymph node. 4. 22 Stage IIE-2: abdominal, but beyond perigastric, lymph nodal involvement.

- Patient must have signed the informed consent and agree to provide achieved

pathologic material for immunohistochemical study and for RT-PCR t(11;18)(q21;q21) determination.

Exclusion Criteria:

- Patients with extensive gastrointestinal tract involvement are not eligible.

- Patients with previous history of extranodal lymphoma are not eligible.

- Patients with stage IIE-2 or beyond disease: infiltration of regional lymph node,

e. g. paraaortic, renal hilar, retroperitoneal, mesenteric, or lymph node of gastrosplenic ligament and of hepatoduodenal ligament; or involvement of lymph node above and below diaphragm (Stage III) or other visceral organ involvement (stage IV) are not eligible.

- Patients with cardiopulmonary status that do not allow repeat endoscopy are not

eligible.

- Patients with prior antibiotics, chemo- or radiotherapy for their gastric lymphoma

are not eligible.

- Patients who had previous anti-H. pylori therapy and without pretreatment pathology

achieve material for histological review and immunohistochemical study are not eligible.

Wei-lien Feng, RN,MSN, Phone: 886-2-8792-3311, Ext: 17644, Email: winnif@nhri.org.tw

China Medical University Hospital, Taichung 40447, Taiwan; Recruiting
Ya-Lin WU, B.S., Phone: 886-4-22052121, Ext: 5372, Email: yalin@nhri.org.tw
Chang-Fang Chiu, M.D., Principal Investigator

Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan; Recruiting
Wei Te-Chih, B.S, Phone: 886-7-3121101, Ext: 6109, Email: hemawei@nhri.org.tw
Wen-Ming Wang, Principal Investigator

Mackay Memorial Hospital, Taipei 104, Taiwan; Recruiting
Mei-Wha Lee, B.S., Phone: 886-2-2543-3535, Ext: 3454, Email: lmh@nhri.org.tw
Chan-En Wang, M.D., Principal Investigator

National Taiwan University Hospital, Taipei 112, Taiwan; Recruiting
Bor-Rong Chen, B.S., Phone: 886-2-2312-3456, Ext: 7677, Email: brong@nhri.org.tw
Li-Tzong chen, M.D. PhD, Principal Investigator

National Taiwan University Hospital, Taipei 115, Taiwan; Recruiting
Ling-Fang Lin., B.S., Phone: 886-2-2312-3456, Ext: 7677, Email: dale@nhri.org.tw
Chi-An Chen, M.D., Principal Investigator

Tri-Services General Hospital, Taipei 11490, Taiwan; Recruiting
Tsai Yuan Shieh, M.D, Ph.D, Principal Investigator

Veterans General Hospital-Taipei, Taipei 112, Taiwan; Recruiting
Hsueh-Pin Yu, B.S., Phone: 886-2-2871-2121, Ext: 2518, Email: dhpyu@nhri.org.tw.
Tzeng Shing Chen, M.D., PHD, Principal Investigator

Starting date: July 2006
Ending date: December 2014
Last updated: September 14, 2006