Inflammatory Cytokines Profile in Individuals Subjected to Surgical Procedures Using Propofol or Isoflurane
Information source: UPECLIN HC FM Botucatu Unesp
ClinicalTrials.gov processed this data on August 23, 2015 Link to the current ClinicalTrials.gov record.
Condition(s) targeted: Patients in Good Health
Intervention: Propofol and isoflurane (Drug)
Phase: Phase 4
Status: Completed
Sponsored by: UPECLIN HC FM Botucatu Unesp Official(s) and/or principal investigator(s): Marina Á Mazoti, MsC, Principal Investigator, Affiliation: Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP
Summary
The aim of this study is to assess the profile of cytokines IL-1beta, IL-6, IL-8, IL-10,
IL-12 and TNF-alpha under inhalation anesthesia with isoflurane and intravenous anesthesia
with propofol in healthy patients subjected to minimally invasive elective surgeries.
Clinical Details
Official title: Inflammatory Cytokines Profile in Individuals Subjected to Surgical Procedures Using Propofol or Isoflurane.
Study design: Allocation: Randomized, Endpoint Classification: Pharmacodynamics Study, Intervention Model: Parallel Assignment, Masking: Double Blind (Subject, Investigator), Primary Purpose: Supportive Care
Primary outcome: Evaluation of cytokines profile
Secondary outcome: Evaluation of pro and anti-inflammatory cytokines
Detailed description:
Twenty ASA-I patients, subjected to otorhinolaryngology surgery, were randomly allocated in
one group to receive anesthesia with isoflurane 1 MAC (minimum alveolar concentration) (n =
20). Other group with twenty ASA-I patients received propofol 2 to 4 microgram mL-1 (n =
20).
Fentanyl 5 mg kg-1 and rocuronium bromide 0. 6 mg kg-1 were also administered to all
patients. Venous blood (10 mL) was collected from each patient at each of the following
times: before the beginning of surgery and anesthesia (T1), 2 h after the beginning of
surgery (T2), and on the day after the anesthetic-surgical procedure (T3).
Plasma concentrations of interleukins IL-1beta, IL-6, IL-8, IL-10 and IL-12 and tumor
necrosis factor (TNF-alpha) were measured in each sample through flow cytometry technique by
using the method Cytometric Bead Array (CBA). Venous blood samples from fifteen volunteers
not subjected to stress were also collected as control, and the same cytokines were
measured.
Eligibility
Minimum age: 18 Years.
Maximum age: 50 Years.
Gender(s): Both.
Criteria:
Inclusion Criteria:
- Patients in good health American Society of Anesthesia status physical I
- Elective minor surgery
- General anesthesia
Exclusion Criteria:
- Smokers
- Alcoholics
- Previous medication or radiation
Locations and Contacts
Botucatu Medical School, UNESP, Botucatu, São Paulo 18603-970, Brazil
Additional Information
Starting date: March 2008
Last updated: April 26, 2010
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