Study of Propranolol as Anti-Adhesive Therapy in Sickle Cell Disease (SCD)
Information source: Duke University
ClinicalTrials.gov processed this data on August 23, 2015
Link to the current ClinicalTrials.gov record.
Condition(s) targeted: Sickle Cell Disease
Intervention: Propranolol (Drug); Placebo (Drug)
Phase: Phase 2
Sponsored by: Laura M. De Castro, MD
An open label, prospective, randomized cross-over phase II study in up to 60 sickle cell
patients who are either homozygous for Hb S or have HbSB0 thalassemia. Initially, each
patient will be treated for 6 weeks with placebo or a standard dose of propranolol (40 mg)
every 12 hrs. This will be followed by a 2-week washout period after which, patients will
receive the other treatment modality (placebo or propranolol).
We Hypothesize that propranolol administered in vivo on a daily basis for 6 weeks (1) will
decrease baseline adhesion to endothelial cells and will substantially abrogate
epinephrine-stimulated adhesion to endothelial cells, as measured in vitro; (2) will improve
biomarkers of endothelial activation and dysfunction; and (3) can be safely used in patients
with SCD. Thus, the use of propranolol in SCD may represent a safe and effective means of
anti-adhesive therapy in SCD.
• To establish the safety and efficacy of long-term therapy with propranolol as an
anti-adhesive therapy for SCD.
• To evaluate changes in soluble markers of endothelial activation and dysfunction.
Correlative Science Objective:
• To determine whether response to propranolol therapy is associated with polymorphisms in
genes encoding the proteins involved in the upregulation of Sickle Red Blood Cell (SS RBC)
adhesion by epinephrine.
Official title: Phase II Study of Propranolol as Anti-Adhesive Therapy for Sickle Cell Disease
Study design: Allocation: Randomized, Endpoint Classification: Safety/Efficacy Study, Intervention Model: Crossover Assignment, Masking: Double Blind (Subject, Caregiver, Investigator), Primary Purpose: Treatment
SS RBC Adhesion (Epi -1d/cm2- vs. Sham) by Treatment
SS RBC Adhesion (Epi -2d/cm2- vs. Sham) by Treatment
SS RBC Adhesion (Epi -3d/cm2- vs. Sham) by Treatment
Overall Change of Plasma Levels of sE-selectin
Overall Change of Plasma Levels of sP-selectin
Overall Change of Plasma Levels of sICAM-1
Overall Change of Plasma Levels of sVCAM-1
Overall Change of Hemoglobin (Hgb) Levels
Overall Change of Hematocrit (Hct) Levels
Overall Change of Lactate Dehydrogenase (LDH) Levels
Overall Change of Oxygen Saturation (02Sat) Levels
Overall Change of Systolic Blood Pressure Levels
Overall Change of Diastolic Blood Pressure Levels
Minimum age: 18 Years.
Maximum age: N/A.
- Diagnosis by electrophoresis (HEP) of Hemoglobin (Hgb) SS or Hgb Sβ0 thalassemia (all
patients followed at our clinic have HEP-confirmed diagnosis on file)
- Age ≥ 18 years
- Blood pressure (BP) Systolic ≥ 95mm Hg and Diastolic ≥ 50mm Hg
- Heart rate (HR) ≥ 70 and ≤ 110 bpm
- Oxygen saturation by pulse oximeter and at room air ≥ 92%
- Hematocrit (Hct) ≥ 20% and Hb > 6. 0 g/dL
- Euthyroid status as indicated by normal Thyroid Stimulating Hormone (TSH)
- SS RBCs obtained during screening period demonstrating an adhesion response to
epinephrine of 40% over non-stimulated baseline adhesion to endothelial cells
- Capacity to understand and sign informed consent
- History of vaso-occlusive episode during the 6 wks prior to screening
- RBC transfusion during the 3 months prior to study entry
- Ongoing pregnancy
- History of heart failure, myocardial infarct (MI), bradyarrhythmias, conduction
- History of asthma or reactive airway disease
- History of thyroid disease
- Renal insufficiency (BUN >21 mg/dL and/or Creatinine >1. 4 mg/dL)
- Use during the screening or study period of any of the following medications:
antihypertensives, diuretics, thyroid replacement therapy, anti-arrhythmia
medications, bronchodilators, inhaled steroids, insulin, or hypoglycemic medication
- History of allergy to sulfonamides
Locations and Contacts
Duke University Medical Center, Durham, North Carolina 27710, United States
Zennadi R, Hines PC, De Castro LM, Cartron JP, Parise LV, Telen MJ. Epinephrine acts through erythroid signaling pathways to activate sickle cell adhesion to endothelium via LW-alphavbeta3 interactions. Blood. 2004 Dec 1;104(12):3774-81. Epub 2004 Aug 12.
Zennadi R, Moeller BJ, Whalen EJ, Batchvarova M, Xu K, Shan S, Delahunty M, Dewhirst MW, Telen MJ. Epinephrine-induced activation of LW-mediated sickle cell adhesion and vaso-occlusion in vivo. Blood. 2007 Oct 1;110(7):2708-17. Epub 2007 Jul 3.
Zennadi R, Chien A, Xu K, Batchvarova M, Telen MJ. Sickle red cells induce adhesion of lymphocytes and monocytes to endothelium. Blood. 2008 Oct 15;112(8):3474-83. doi: 10.1182/blood-2008-01-134346. Epub 2008 Jul 29.
Eyler CE, Jackson T, Elliott LE, De Castro LM, Jonassaint J, Ashley-Koch A, Telen MJ. beta(2)-Adrenergic receptor and adenylate cyclase gene polymorphisms affect sickle red cell adhesion. Br J Haematol. 2008 Apr;141(1):105-8. doi: 10.1111/j.1365-2141.2008.07008.x.
De Castro LM, Zennadi R, Jonassaint JC, Batchvarova M, Telen MJ. Effect of propranolol as antiadhesive therapy in sickle cell disease. Clin Transl Sci. 2012 Dec;5(6):437-44. doi: 10.1111/cts.12005. Epub 2012 Oct 17.
Starting date: June 2010
Last updated: January 19, 2015