The primary objective of this study is to determine the effect of repeat oral doses of
eliglustat 150 mg twice daily (BID) (or 100 mg BID for CYP2D6 poor metabolizers) on the
pharmacokinetics (PK) of orally administered digoxin 0. 25 mg in healthy adult subjects. This
will be a single-site, open-label study in 2 staggered cohorts of healthy adult subjects.
The study will comprise a screening period (between Day - 45 and Day -2), treatment period 1
(Day - 1 to Day 4), treatment period 2 (Day 11 to Day 18), and a safety follow-up visit (Day
24 ± 1). There will be a 10-day washout between dosing of study drug in Period 1 and Period
2. The duration of each subject's participation in the study, inclusive of the screening and
follow-up visits, will be approximately 10 weeks.
2. In each cohort, eligible subjects will be admitted to the clinical facility on Day - 1,
the day prior to the start of dosing in Period 1, and will remain in the clinic until
completion of study procedures on Day 18, the last day of Period 2 (an approximately
18-night inpatient stay). In Period 1, all subjects will receive a single oral dose of
digoxin 0. 25 mg on Day 1. In Period 2, all subjects will receive repeat oral doses of
eliglustat 150 mg BID (or 100 mg BID if a CYP2D6 poor metabolizer) from Day 11 to Day 17 and
a single oral dose of digoxin 0. 25 mg on Day 15. For Cohort 2, co-administration of digoxin
and eliglustat on Day 15 will occur only after review of safety data (including telemetry
data) through at least Day 16 for all subjects in Cohort 1. The decision to proceed with
concomitant dosing of subjects in Cohort 2 will be at the discretion of the Investigator,
with consultation of the Sponsor, as appropriate.
Minimum age: 18 Years.
Maximum age: 45 Years.
- The male or female subject is in good general health
- The subject has a body weight of 50 to 100 kg (110 to 220 lb) with a body mass index
(BMI) â‰¤32 kg/m2 at screening.
- The subject's physical examination, laboratory, vital sign, and electrocardiogram
(ECG) test results are within normal limits at screening and Day - 1 or, if abnormal,
are not considered clinically significant in the opinion of the Investigator.
- The subject has been a non-smoker for at least 6 months prior to the time of
providing informed consent, and is willing and able to abstain from smoking (and use
of other forms of nicotine) until completion of the safety follow-up visit.
- The subject has not used drugs of abuse for at least 6 months prior to Day -1 and is
willing and able to abstain from using drugs of abuse until completion of the safety
- The subject is willing and able to abstain from alcohol for 48 hours prior to the
first dose of study drug until completion of the safety follow-up visit.
- The subject is willing and able to abstain from grapefruit and grapefruit juice for
72 hours prior to the first dose of study drug until completion of the safety
- The subject is willing and able to maintain a normal-fiber diet (i. e., to abstain
from excess fiber-rich foods) for 72 hours prior to the first dose of study drug
until completion of the safety follow-up visit.
- Female subjects of childbearing potential must have a documented negative pregnancy
test at screening and Day 1, and be willing to use a medically accepted form of
contraception (as defined in the protocol) from screening until 30 days after the
last dose of study drug. A woman of childbearing potential is defined as any female
who has not been amenorrheic for at least 2 years or has not undergone a hysterectomy
or surgical sterilization.
- The subject has any of the following: Clinically significant coronary artery disease
including history of myocardial infarction or ongoing signs or symptoms consistent
with cardiac ischemia or heart failure; clinically significant arrhythmias or
conduction defect such as 2nd or 3rd degree atrioventricular (AV) block, a PR
interval â‰¥210 msec, complete bundle branch block, prolonged QTc interval (e. g.,
repeated demonstration of a QTc interval â‰¥450 msec), or sustained ventricular
- The subject has received antibiotics for any reason within 30 days prior to the first
dose of study drug.
- The subject has received any other prescription or non-prescription medication (with
the exception of nonprescription-strength ibuprofen and acetaminophen) or dietary or
herbal or fiber supplement within 30 days or 5 half-lives (whichever is longer) prior
to the first dose of study drug without the approval of the Investigator and Genzyme.
- The subject receives an immunization within 30 days of providing informed consent.
- The subject has a history of hypersensitivity to digoxin or other digitalis
glycosides, or has other drug allergies that are clinically significant in the
opinion of the Investigator (e. g., significant rash or hives).
- The subject has a clinically significant organic disease, including cardiovascular,
renal, hepatic, gastrointestinal, pulmonary, neurologic, endocrine, metabolic, or
psychiatric disease, or other medical condition such as electrolyte disorders,
serious intercurrent illness, or extenuating circumstance that, in the opinion of the
Investigator, precludes participation in the trial.
- The subject has digestive disorders, including malabsorption, gastroenteritis,
pancreatitis, constipation, gastroesophageal reflux disease, diverticulitis,
irritable bowel syndrome, or inflammatory bowel disease (including Crohn's disease).
- The subject has had a cholecystectomy.
- The subject has a screening laboratory test result >2x the upper limit of normal
(ULN) for any of the following liver function tests: aspartate aminotransferase
(AST), alanine aminotransferase (ALT), gamma glutamyltransferase (GGT), and total
- The subject tests positive for human immunodeficiency virus (HIV) antibody, hepatitis
C antibody, or hepatitis B surface antigen at screening.
- The subject tests positive for urine drugs of abuse, urine alcohol, or urine cotinine
- The subject received an investigational product within 30 days prior to providing
informed consent or plans to receive any other investigational product at any time
during the course of this study.
- The subject donated blood or blood products within 30 days prior to providing
- The subject's schedule or travel plans prevent the completion of all required visits.
- The subject is scheduled for inpatient hospitalization, including elective surgery
(inpatient or outpatient), during the study.
- The subject has a history of cancer, with the exception of basal cell carcinoma.
- The female subject of childbearing potential is pregnant or lactating.
- The subject, in the opinion of the Investigator, is unable to adhere to the
requirements of the study.