[Effects of urokinase on renal interstitial fibrosis and transforming growth factor-beta1 in the kidney of rats with chronic cyclosporine A nephropathy]

Author(s): Wang Y, Tong JR, Luo ZM, He F, Ma L

Affiliation(s): Deparment of Nephrology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. yinwang138@126.com

Publication date & source: 2009-12, Nan Fang Yi Ke Da Xue Xue Bao., 29(12):2449-52.

Publication type: English Abstract

OBJECTIVE: To investigate the effects of urokinase on renal interstitial fibrosis and transforming growth factor-beta1 (TGF-beta1) in the kidney of rats with chronic cyclosporine A nephropathy. METHODS: Male Sprague-Dawley rats on low-salt diet were randomly divided into control (VH), CsA-treated (CsA), CsA+2000 U/kg.day uPA (CsA+U2) and CsA+6000 U.kg.3 days (CsA+U6) groups. The rats were given CsA intragastrically for 4 weeks to prepare CsA-induced chronic nephropathy model. Masson staining was used to examine fibrin deposition. Western blotting and reversal transcription polymerase chain reaction were employed to evaluate urokinase-type plasminogen activator (uPA) and TGF-beta1 protein and gene expressions, respectively. RESULTS: CsA can increase fibrin deposition and the expression of TGF-beta1 in the renal tissue, which were significantly reduced after uPA treatment (P<0.05). CONCLUSION: Continuous low-dose uPA treatment can reduce renal interstitial fibrosis in rats poosibly in association with its inhibitory effect on TGF-beta1 expression.