Skeletal muscle is anabolically unresponsive to an amino acid infusion in pediatric burn patients 6 months postinjury.
Author(s): Tuvdendorj D, Chinkes DL, Zhang XJ, Sheffield-Moore M, Herndon DN
Affiliation(s): Department of Surgery, University of Texas Medical Branch Galveston, TX, USA.
Publication date & source: 2011-03, Ann Surg., 253(3):592-7.
Publication type: Randomized Controlled Trial; Research Support, N.I.H., Extramural
OBJECTIVE: To evaluate leg muscle, whole-body muscle, and whole-body nonmuscle protein response to anabolic signaling of amino acids in pediatric burn patients at 6 months after injury. BACKGROUND: Burn injury is associated with a catabolic state persisting years after the injury. The tissue response to nutritional signaling (eg, amino acids) plays a critical role in tissue protein net balance via coordination of protein synthesis and breakdown mechanisms. METHODS: A total of 10 patients (7.4 +/- 3.8 years; 27.4 +/- 14.7 kg) and 5 healthy young males (22 +/- 3 years; 76 +/- 15 kg) underwent an 8-hour stable isotope infusion study. During the last 3 hours, an amino acid solution (10% Travasol, Clintec Nutrition, Deerfield, IL) was infused. Femoral arterial and venous blood samples and muscle biopsy samples were collected throughout the study. A P value of less than 0.05 was considered statistically different. RESULTS: During amino acid infusion, leg muscle protein synthesis rate significantly increased (P < 0.05) in both groups, however, in the burn group, protein breakdown also increased, although nonsignificantly. As a result, protein net balance remained negative. In the control group, breakdown nonsignificantly decreased resulting in a significant increase (P < 0.05) in muscle protein net balance. Whole-body protein breakdown was significantly higher in the burn patients. CONCLUSION: In pediatric burn patients at 6 months postinjury, leg muscle protein net deposition is unresponsive to amino acid infusion; and whole-body protein breakdown is significantly higher than in the control group.