Synthesis and biological evaluation of the suberoylanilide hydroxamic acid (SAHA) beta-glucuronide and beta-galactoside for application in selective prodrug chemotherapy.

Author(s): Thomas M, Rivault F, Tranoy-Opalinski I, Roche J, Gesson JP, Papot S

Affiliation(s): UMR-CNRS 6514, Synthese et Reactivite des Substances Naturelles, Universite de Poitiers, 40, Av. du Recteur Pineau, 86022 Poitiers, France.

Publication date & source: 2007-02-15, Bioorg Med Chem Lett., 17(4):983-6. Epub 2006 Nov 17.

Publication type: Research Support, Non-U.S. Gov't

The beta-O-glucuronide and beta-O-galactoside of SAHA have been prepared and evaluated as prodrugs for selective cancer chemotherapy (ADEPT, PMT). These new compounds are stable under physiological conditions and do not exhibit any antiproliferative activity compared to the parent drug after a 48-h treatment of H661 cells. The glucuronide derivative did not lead to the release of the drug in the presence of either Escherichia coli or bovine liver beta-glucuronidase. On the other hand, under enzymatic cleavage of galactoside prodrug by the corresponding enzyme, a rapid release of SAHA was observed demonstrating that the beta-O-galactoside of SAHA is a promising candidate for in vivo investigations.