Effect of dietary purines on the pharmacokinetics of orally administered ribavirin.
Author(s): Li L, Koo SH, Limenta LM, Han L, Hashim KB, Quek HH, Lee EJ
Affiliation(s): Department of Pharmacology, National University of Singapore, Clinical Research Centre, Blk MD11, #05-9, 10 Medical Drive, Singapore 117597.
Publication date & source: 2009-06, J Clin Pharmacol., 49(6):661-7. Epub 2009 Apr 15.
Publication type: Randomized Controlled Trial; Research Support, Non-U.S. Gov't
Ribavirin is found to be absorbed in the intestine through the human concentrative nucleoside transporter 2 (hCNT2). Cellular uptake of ribavirin was strongly inhibited by purine nucleoside in an in vitro study. This study aims to examine the effects of dietary purine on the pharmacokinetics of orally administered ribavirin in vivo. Twenty healthy participants were enrolled in a randomized, 2-period crossover study. Participants were administered a single 600-mg oral dose of ribavirin after either a high-purine meal or a low-purine meal. Serial blood samples were collected predose and over 144 hours after dosing. Ribavirin concentrations were measured by liquid chromatography/tandem mass spectrometry. In comparison with corresponding plasma values of ribavirin following a high-purine meal, C(max), AUC(0-144) and AUC(0-infinity) of ribavirin following a low-purine meal were 136% (90% confidence internal [CI]: 120%-155%), 134% (90% CI: 118%-153%), and 139% (90% CI: 120%-159%), respectively. This study indicates that dietary purines have an effect on ribavirin absorption. Dosage regimens of ribavirin might need to be adjusted according to the purine content of the meal.