[Determination of five components in compound hypotensive tablet by HPLC]

Author(s): Che BQ

Affiliation(s): Beijing Institute for Drug Control, Beijing 100035, China. chebaoquan89@sina.com

Publication date & source: 2004-08, Yao Xue Xue Bao., 39(8):618-20.

AIM: To establish a method for the determination of the five components (reserpine, chlordiazepoxide, hydrochlorothiazide, dihydralazine sulfate, triamterene) in compound hypotensive tablet. METHODS: The chromatography was performed using a CN column with acetontrile-0.1 mol L(-1) sodium heptasulfonate solution (7:3) and (5:5) as the mobile phases. The detection wavelength was 267 nm for reserpine, chlordiazepoxide and hydrochlorothiazide, 310 nm for dihydralazine sulfate, 360 nm for triamterene. RESULTS: The linear range of each component was tested, and the recovery and stability of each component was satisfactory, three lots of samples were determined using the method. CONCLUSION: This is an accurate and credible quality control method for compound hypotensive tablet.