Plasma and salivary amoxicillin concentrations and effect against oral microorganisms.

Author(s): Baglie S, Del Ruenis AP, Motta RH, Baglie RC, Franco GC, Franco LM, Rosalen PL, Silva P, Groppo FC

Affiliation(s): Department of Pharmaceutical Sciences, Ponta Grossa State University, Ponta Grossa, PR, Brazil.

Publication date & source: 2007-10, Int J Clin Pharmacol Ther., 45(10):556-62.

Publication type: Randomized Controlled Trial; Research Support, Non-U.S. Gov't

Plasma and salivary amoxicillin (AMO) concentrations were quantified following a single oral dose (875 mg) of two formulations of AMO (Amoxicillin-EMS Sigma Pharma and Amoxil BD 875 mg). In addition, the effect of amoxicillin against oral microorganisms was accessed. The open, randomized, two-period crossover study was carried out in 20 volunteers. Saliva and blood samples were collected at 0, 0.5, 1, 2, 4, 8 and 12 h after drug administration, and quantified using HPLC-ESI-MS and HPLC, respectively. Streptococci counts, anaerobe counts and total microorganism counts were obtained. No differences were observed between formulations (p > 0.05) in the plasma and salivary AMO concentrations and the pharmacokinetic parameters (C(max), t(max), AUC(0-8), and AUC(0-infinity)) also showed no statistically significant differences between formulations (p > 0.05). Microorganism counts for the two formulations at all sampling times did not differ (p > 0.05) but all microorganism counts at 60 min post-dose showed a significant decrease (p < 0.05). Amoxicillin was effective in reducing oral microorganism levels up to 12 h post-dose.